The purpose of this study was to investigate the conditional change of bio-energy as a result of Chen style Tai-Chi Chuan(CSTCC) of old form, first sort, and combine 83 steps movement with a case study. In this case study, the subject who practiced CSTCC 3.-5 times per week and had age of 43 years; height of 167 cm; weight of 63.5 kg, and practice ages of 6 years. The focus of analysis was on the twenty-four key meridian points in human body. The Ryodoraku theory by Yoshio Nakatani was chosen as the theoretical basis for investigation. This integration enabled us to automatically calculate, analyze, and judge the conditional change of before practice and after practice 5, 10, 20 minutes in bio-energy value, which was a reflection of the meridian phenomena. An Excel statistic analysis was cnducted to identify the conditional change between prior practice and post practice 5, 10, 20 minutes of CSTCC. The result indicated that for the prior practice, the bio-energy value was significantly lower than that of the post practice. Additionally, the meridian electric resistance decreased. And then the value of bio-energy decreased as the resting times. This phenomenon is related to the vitalized neural transfer mechanism and smoothed neural electricity, which are triggered by Tai Chi Chuan exercises, these exercises manage to bring up intracellular neural transfer and cause the polarization of cell membrane. The research findings suggest that CSTCC practicing enables metabolism improvement and contributes to smooth blood circulation. The results provide implications for health preservation science.