Purpose: The purpose of this study is to design a dilation nail to improve the fixation strength of autograft tendon for anterior cruciate ligament reconstruction. Methods: Four new design dilation nails were manufactured and were implanted into pig knee joint by simulating anterior cruciate ligament reconstruction surgery. One interference screw was used as a control group, two dilation nails was implanted into proper position and the other two dilation nails was implanted as simulating malposition. The pig knee joint was mounted onto the material testing system and the maximum pull out strength was record to evaluate the fixation strength of the new dilation nail and the interference screw fixation methods. Results: Two new design dilation nails with proper implanted were failure due to autograft ligament torn and their maximum pull out strength were larger than the control group. The other two new design dilation nails with malposition implanted were failure on the fixation position that had the similar maximum pull out strength comparing with control group. Conclusions: From this study, we found the fixation strength of new design dilation nail is greater than the control interference screw group. It is revealed the feasibility of this new design in clinical application.