Objectives: A geographic information system (GIS) was used to explore the medical accessibility of each administrative unit in Kaohsiung city. Methods: ArcGIS was used to gain the information regarding the population and transportation distance of each grid, and sort the information through cluster analysis. Results: The rank-four areas, which account for 3% of the entire sample in Kaohsiung city, are the most convenient areas to receive medical service. The rank-three areas are the second most convenient areas to receive medical service, accounting for 66% of the entire sample in Kaohsiung city. The rank-one and two areas, however, have poor medical accessibility and account for 31% of the entire sample in Kaohsiung city, most of which are located northeast of Kaohsiung city. In addition, the villages which should be a focus of concern and reinforced with respect to medical accessibility in the future are listed. Conclusions: This research provids a detailed viewpoint of the division of Kaohsiung city based on different medical accessibility ranks, and further assists authorities to implement related policies and practices in the future.