The purposes of this study were to investigate knowledge, attitudes and their related factors toward chemotherapy among nurses and the relationship between knowledge and attitudes. 215 nurses working in a medical center in central Taiwan were selected by random sampling. 196 questionnaires form the basis for data analysis. The results were: 1. The average score was 55.6 on a 100 point scale, 66.3 % of nurses had taken the courses for chemotherapy at school, 6.1 % of nurses attended chemotherapy semminar, 84.7% of nurses were not satisfied with chemotherapy courses arranged by hospital. 2. The highest average score of knowledge toward chemotherapy was the item of administering drug safetly and prevention intervention (62.4%), the lowerest score of knowledge was the item of drugs of chemotherapy. 3. There were significant differences in knowledge toward chemotherapy among different educational backgrounds, depending on whether or not nurses had attended a chemotherapy semminar, and on different working units (p<.05). 4. The most frequently cited attitude toward chemotherapy was the attitude toward clinical application of chemotherapy (69.8 %). The other attitudes were side effects of chemotherapy and nursing care (69.6 %), administering drugs safetly and prevention intervention (68.8%), chemotherapy drugs (65.3%), ability and faith in care of patients receiving chemotherapy (61.6%), and perception of chemotherapy (56.8%) respectively. 5. The nurses who had experience in cancer nursing and chemotherapy ,whose relatives have had chemotherapy , and had attended a semminar before, had a more positive attitude toward chemotherapy. 6. The major three causes of nurse not being willing to care for patients receiving chemotherapy were: fear of injury from chemotherapy, side effects of chemotherapy, emotion of nurses affected by chemotherapy. 7 There was a statistically significant, positive relationship between knowledge and attitude (p<.05).