Objectives: This study examined the status and factors related to nurses' knowledge, attitudes, and behaviors toward the life-sustaining treatment choices of terminally ill patients to provide a reference for the relevant authorities who formulate policies and strategies for enhancing the quality of health care services. Methods: A sample of nurses from four hospitals in central Taiwan was investigated. A purposive sampling method was used to obtain cross-sectional data through a self-administered structured questionnaire. From a total of 564 questionnaires that were administered, 465 valid questionnaires were collected; this yielded a valid response rate of 82.4%. Data were analyzed using SPSS version 17.0. Results: A significantly moderate positive correlation was observed among the nurses' knowledge, attitudes, and behaviors toward certain life-sustaining medical treatments. Of all independent variables, being 20-29 years of age, attending an average of 1-hour palliative medical service training course annually, possessing adequate knowledge of life-sustaining medical treatments, and having a positive attitude considerably correlated with nurses' behaviors toward life-sustaining medical treatments. The model explained 43.5% of the variance in nurses' behaviors toward life-sustaining medical treatments. Nurses' knowledge of life-sustaining medical treatments exhibited the highest correlation, thus explaining their behaviors through the partially mediating effect of their attitudes. Conclusions: Nurses should periodically examine the status and factors related to their knowledge, attitudes, and behaviors toward terminally ill patients' choices of life-sustaining medical treatments to increase their knowledge and skills, enhance their positive attitudes, and execute life-sustaining medical practices through on-the-job education. These efforts may protect the dignity of terminally ill patients and improve the quality of life-sustaining medical care services.