Ancestor worship ritual in ancestral hall is an important ritual for traditional clan to deepen its blood identity and maintain living order.From the startup of the gentry’s worship ritual model of Yi Li(the Book of Etiquette and Ceremonials)in pre-Qin Dynasty to the inheritance and changing practice in Song Dynasty and Ming Dynasty to Jia Li(Family Ritual),women’s role in the ancestor worship ritual is marginalized from housewives in a core position to kitchen maids for preparing the sacrifices.The historical changes of women’s sacrificial role indicate that the seasonal holding of ancestor worship ritual in ancestral hall is the maintenance and strengthening of the gender order and the feudal ethics in clan life.