A beautiful scene not only diverts travelers’ mood but also enables them to understand the environment along roadsides. The section of investigated road was from the Taichung Harbor to Kang-Sen interchange of Sun-Yi-Sen Highway. By reviewing the references of environmental perception, environmental preference, and visual assessment, we conducted the research method. Firstly, the types of the landuse along Sun-Yi-Sen Highway were extracted by factor analysis, there were eight types: farmland, artificial facilities, low-density residence, high-density residence, factories, landmarks, and cultivated plants. Legibility, complexity, coherence and mystery were selected as four evaluated criteria. Then the processes of perception of travelers toward the landuse types along roadsides and the difference of landscape preference due to the different socio-demographic backgrounds of users were analyzed. The results of this investigation show: (1)the more natural landscape is the more attracted to traveler; (2)the evaluated criteria reveal complex relationships among the preference and landuse types. The farmland has a great difference with the other landuse types. (3)The socio-demographic variables have less predictive ability than psychological evaluated variables. There are suggestions provided to the land use management along the Sun-Yi-Sen Highway and the considerations of road-line selection.