The questionnaire survey which was conducted in PE teaching and students' learning interests is a useful measurement tool in survey research. The researcher not only needs to care for questionnaire’s reliability and validity, but also have to determine appropriate sample sizes for the study. In order to calculate appropriate sample size of the questionnaires, we must first determine the error estimation, margin of error, confidence level values, and the nature of the questionnaire which is continuous or categorical data. If it is continuous data, then we need to decide whether a five-point or seven-point scale to be used. Moreover, we need to consider the returning rate, oversampling size, and uncooperative subjects.