Due to the increase of tourists in the Kenting National Park in recent years, the stability of its ecological environment has been influenced by human activities. For the purpose of sustainable development of the national park, it is very important to effectively monitor the transition of land cover and to efficiently explore the influences on the changes of landscape ecology. To achieve the above objective, this research first utilized spatial technologies, including remote sensing and geographic information system, to gather data of the transition of the Kenting area in the past 10 years. The FRAGSTATS software was then employed to calculated the indices of landscape ecology. The resultant indices and corresponding field work were finally used to analyze the changes of landscape ecology and their impacts on the Kenting National Park. Based on the literature reviews, we determined that the year of 1999 was the key time of land cover changes. For this reason, three SPOT images dated 1994 (January 4), 1999 (April 28) and 2004 (April 21) were utilized. In addition, two aerial photographs (1997 and 2002) and data acquired by the field work were used for the accuracy assessment of the classified images. The analysis of the overall landscape ecology indices demonstrated that Kenting National park is quite balanced. After further examination on various indices, we found that the forest regions near mountain areas have become larger and more compact. The living space for the species in Kenting is therefore getting larger and more stable. From the examination of satellite images, landscape ecology indices and field survey showed that more and more hotels and recreation facilities are built along the coastal areas and famous tourist attractions. This situation will cause negative influences on the animals and plants near the marine ecology and scenic sots in Kenting.