The research aims to discuss the implications of tiao-i in Guqin music. Following an introduction, the author begins with reviewing previous research, examining and evaluating their individual opinions as a literature review and a discussion for this study. With reference to the 33 Guqin handbooks collected in ”Qin Qu Ji Cheng” ('Grand Anthology of Guqin Music') which were published by the Zhonghua Book Company in Beijing, the author will reexamine the use of tiao-i. Furthermore, the author will refer to the statements made by Chen, Min-zi during the Yuan dynasty. Then the author will explore the role of tiao-i in the Chinese Guqin handbooks from the composition theory of Guqin, and take the significance of the ”Ruibin Tune”, ”Yu-Ko Tune” and ”Yu-Ko” which were collected in ”Zhe Yin Shi Zi Qin Pu” as evidence. Finally, the author will present the multiple meanings and functions of tiao-i. Most specifically, how tiao-i indicates the style of Guqin music will also be investigated.