A fictitious case was designed in the pre-test to collect the heart rate of 66 volunteers as honest examinees. These basic data were used to compare with the heart rate variation in the post-test. In the post-test, 32 examinees were assigned in an experimental group (deceptive) and 34 examinees were in a control group (honest) at random. According to statistical data of heart rate variation of the pre-test and the post-test, the experimental group heart rate increased 5.25 beats per minute, but the control group heart decreased 3.47 beats per minute, and it is significant difference between 2 groups(p=0.000). Besides, the heart rate of test beginning is the best window to exhibit the subject emotion in the polygraph test from counting different zone heart rate changes. In the examinee evaluate pressure vibration of the pre-test and the post-test mentioned analysis, there is no significant difference between the experimental group and control the group in pre-test pressure vibration (p=0.869), but significant difference (p=0.000) in the post-test. This suggests that the circumstances control is ideal and the experiment result is useful in the field case.