The aim of this study was to investigate the effect of physical fitness included the over quotas of 12-year Basic Education for public senior and vocational high school students. Research methods of the study were physical fitness performance and questionnaire survey. The physical fitness performance of subjects had 3574 included 1836 from 2013 and 1738 from 2014, they were the first graders of public senior and vocational high schools in Hualien city. The fitness original data uses the physical fitness norm-referenced form Ministry of Education and then perform data processing and analysis with SPSS 20.0. The questionnaire survey of subjects were the first graders of public senior and vocational high schools in Hualien city from 2014 and then proceeded sampling in the research population. Total 410 copies of questionnaires are distributed and 403 copies are retrieved, in which 390 valid copies make the availability ratio of 95.1%. The retrieved questionnaires are proceeded by descriptive statistics and t-test with SPSS 20.0. The results of this study showed as follows: 1. 12-year Basic Education policy intervene the course of physical fitness, it really have significant promotion for senior and vocational high school students in Hualien city. 2. The fitness performance of the public senior high school students are better than the public vocational high school students in Hualien city. 3. The fitness performance of the public senior and vocational high school male students are better than female students in Hualien city. 4. There were significant differences among participated i physical fitness included associated knowledge about the 12-year Basic Education n sports club of ""and "personal fitness expectations", participated in sports club is better than doesn't participated in sports club. 5. There were significant differences among personal exercise habits of "physical fitness included associated knowledge about the 12-year Basic Education" and "personal fitness expectations", personal exercise habits more than five times a week have the best performance.