The purpose of the study was to help the Ministry of the Interior investigate what materials of prevention education of sexual assault will attract adolescents’ attention in order for the future design of the curriculum in addition, this study was to explore how the demographics influence students’ responses in the areas of sexual assault prevention knowledge, attitude for sexual assault prevention education, sexually related attitude, sexually related behavior and needs for prevention education of sexual assault. To make the sample representative, 9public junior high schools from northern, central and southern parts of Taiwan were selected in this study. There were 1,000 students participating in this study and 950 valid questionnaires were completed and returned. The results indicated that students had poor knowledge in terms of sexual assault myth and its related prevention resource. The majority of the students held positive tendency toward attitude for sexual assault prevention education and sexually related attitude. In the area of sexually related behavior, having gender discrimination behavior, telling sexual joke and reading pomographic information were the common behaviors amongst the youth group. The result also indicated that the curriculum content the students needed most were identification of the dangerous environment, crisis management and self-protection. From the results, female students performed better in the subscales of sexual assault prevention knowledge, sexually related attitude, attitude for sexual assault prevention education, sexually related behavior and needs for prevention education of sexual assault than their counterparts. Students’ preferred educational presentations were the drama performing, visiting the sexual assault prevention center and participating in group activities. Their favorite materials were videotape, multimedia and overhead format. The results suggested that students were not satisfied with only oral presentation without any teaching aids. Discussion and recommendation were made for the future design of the sexual assault prevention curriculum.