Excess amount of bacteria colonies suspended in indoor air space severely affect indoor air quality (I.A.Q.), which leads to difficulties in epidemic prevention and infection control. Hong Kong explosion causes death flu, in order to avoid extensions gathering to infect a school to suspend classes for two weeks. The air quality control is very important to school environment. As a monitor result through 19 samples, its concentration of bio aerosols in each zone could be arranged a preface: "Activity center"> "Medical">"The student sleep shed" > "Reading room". The average total bacteria and fungi were counted respectively to 1703/1517, 1597/1099, 980/627, 745/450 CFU/m3. Their Specific values were regarded as about 1.12-1.65 in this environment. This research takes various concentrations as 115–500 mgs/L activated chlorine dioxide solution; carry on "a type hand hold to spray fogs" with the comparison of the disinfection effect of "continuous type ultra sound spirit fog". Show both as a result when about 20-25℃ is at the indoor temperature, all the ability is valid for 30 minutes to reduce 24.1% -71.8 % total bacteria. If strengthen a 115 mgs/L chlorine dioxide ultrasonic fog dissemination by 2.5 m/s air flow, also the ability will promote disinfection effect to 91.6 % in 50 minutes. This research result the canning does pursue studies the school environment indoor bio aerosol to fall risk valuation and disinfection program.