Background: Dietary behavior is probably influenced by nutritional knowledge and it has a profound impact on health status. Chronic diseases and obesity have a strong correlation with unhealthy dietary behaviors and nutritional awareness. Purposes: The aim of the study was to investigate the relationships between nutritional knowledge, dietary behavior and health status among university students. Methods: A cross-sectional survey method was used. A total of 557 participants were recruited from a medical university in Hsinchu. The structured questionnaires include the following: demographic characteristics, Instrument of Nutritional Knowledge, Instrument of Dietary Behavior and General Health Questionnaire. Results: (1) Females, 4th-year-university students, nursing students and the participants who have taken nutritional courses before have a higher score of nutritional knowledge and dietary behavior. (2) The overall mean score for nutritional knowledge, dietary behavior and health status is 76.27 (SD=2.12, Range=0-100), 2.95(SD=0.36, Range=1-5), and 2.43(SD=2.71, Range=0-12), respectively. (3) The dietary behavior has a positive correlation with nutritional knowledge (r =.14, p<.01). (4) The participants have a higher score of dietary behavior with a better health status (r = -.15, p<.01). The participants have the better dietary behavior when they have the higher scores of the dietary behavior. Moreover, they have the better health status when they have the lower scores of the health questionnaire. There is a negative correlation between the dietary behavior and the health status. (5) There isn't a significant correlation between nutritional knowledge and health status. Conclusion/Implications for practice: The results of the study shows that nutritional knowledge has a significant impact on dietary behavior. We recommend implementing the nutrition-related courses for all the students at each university.