It is natural that an athlete pursues the goal of outstanding performance and breaking records, especially in professional competitions. The performance of top players is an important target for rear-end players or the players who are keen to join highly competitive contests. For these reasons, this study aimed to investigate the skill performance variation of different levels of female professional golfer by using one-way ANOVA to find out the differences among the different levels of players, which can be the reference resources for the instructors and the players to adjust their training methods. The participants were 140 female professional golfer, who were divided into four grades according to their performance in 2012. The result showed that different grades of players showed significant differences on the skill performance of Greens in Regulation and Putts per Greens in Regulation. Each skill performance of the fourth-grade players significantly fell behind the other three grades of players. The result of this study could be a consultation for the players to make their training plans.