This study aimed to investigate the skill performance variation of different levels of female professional golfers and also discuss the performance of the players who did not follow Greens in Regulation by using one-way ANOVA, which could be the reference resources for the instructors and the players to adjust their training methods. The subjects were 142 female professional golfers, who were divided into four grades according to their Scoring Average in 2013. The results showed that the fourth-grade players significantly fell behind the players of the other three grades on Driving Accuracy, Greens in Regulation, Putts per Greens in Regulation, and Sand Saves, which showed the big gap between the fourth-grade players and the players of the other three grades. On the other hand, there was no significant difference between first-grade and second-grade players on Greens in Regulation and Putts per Greens in Regulation, but there was a significant difference between the performance of both first-grade and the second-grade players who did not follow Greens in Regulation, causing the significant difference on their Scoring Average. The results of this study could be a consultation for the players to make their training plans.