本研究目的在瞭解手術室護理人員工作壓力程度及探討手術室護理人員基本屬性變項對工作壓力之差異。此研究採立意取樣法，以南區某醫學中心手術室護理人員為研究對象，共得111份有效問卷。研究供據乃參考護理人員工作壓力相關文獻及研究者本身工作經驗並訪談多位手術室護理人員所自設而成的「手術室護理人員工作壓力量表」，此量表是以Likert五分量表之結構性問題測量。信度係採內在一致性之檢測，其Cronbach's alpha值為0.92。在效度方面採專家效度鑑定，其結果在適用性上達92.7%。以百分比、平均值、標準差、單因子變異數分析及t檢定等統計方式進行資料之分析。 研究結果發現：手術室護理人員整體工作壓力感受為3.40分（滿分為5分），顯示人員的工作壓力程度介於「尚可至有壓力」之間。在六個工作壓力類別中，手術室的「工作環境」壓力最大，而以「人際關係」最小。在42項工作壓力源中最困擾手術室護理人員的前三項分別是：（1）接觸病患血液及體液時（尖銳物刺傷如：針扎）（2）器官移植捐贈者的遺體護理（3）接觸X－Ray輻射線。另外，在相關影響因素中，「婚姻狀況、有無子女」與否並無顯著差異，但「年齡、教育程度、手術室工作年資及護理職級」與工作壓力類別反應呈顯著差異，其中護理職級以N3人員對行政管理、行政回饋之壓力感受較N1人員大。此研究結果所得可提供手術室護理人員及主管作參考。

　　　　　The purpose of this study was to understand the degree of workplace stress and the relationship between demographic characteristics and stress in operating room nurses. Purposive sampling was used to collect data from 111 operating room nurses at a southern medical center in Taiwan. Literature review and personal experiences were used to design the “Working Stress Questionnaire among Operating Room Nurses (WSQ)” to measure participants' responses. The WSQ is a structured instrument using a five-point Likert scale (5 is the highest score and 1 is the lowest score). The internal consistency reliability for the WSQ is 0.92 (Cronbach's alpha). Expert content validity was 92.7%. Data were analyzed using percentage, mean, standard derivation, one-way ANOVA, and t-test. The results were as follows. The average overall stress scores for operating room nurses was 3.4 which indicating the score represents an acceptable level for a fair testing. Operating room environmental factors were the highest stress sources reported by nurses employed there for 6 months or more. The source of least stress was the relationship among nurses. For the 42 sources of workplace stress, the top 3 were: (1) contact with patients' blood and body fluids during nursing care, such as administering an injection; (2) care of organ donors; and, (3) X-ray exposure. A statistically signigicant difference was found between stress and age, level of education, work experience, and occupational status. No statistically significant difference was found between stress and marital status or between stress and whether or not the nurse had children. Responses were compared based on the hospital's 3-step career ladder. Level 3 nurses (N3) felt more stress about administrative management and feedback than Level 1 nurses (N1). This study implies that nurse leaders and administrators need to develop strategies to help operating room nurses deal with workplace stress.