In accordance with the theory of planned behavior (TPB) from Ajzen (1985), this study purposed two aims: one is to explore the intervention effects of such AIDS-related education as knowledge, attitude, subjective norms, perceived behavioral control, and behavioral intention to take preventive measures on the vocational high school female students. The other is to explore teaching intervention from experimental group. The one-equivalent experimental design was employed in the study to compare the intervening effects between groups. There were totally 494 students participated. Students in the experimental group were intervened by a series of teaching course within a semester. The following conclusions were drawn from the study: (1) Teachers’ influence and exposure to incidence were the significant variables predictable to the students’ preventive behavioral intention. (2) The AIDS preventive behavioral intention is increased with participants’ active attitude and positive subjective norms. The perceived control is relevant to participants’ havioral intention. (3) The intervention program was found effective in altering students’ perception of AIDS. (4) The experimental intervention was also found useful in improving preventive behavior and nursing intention for AIDS medication. (5) The intervention program had a promoting effects in subjects’ normative beliefs both simultaneously and continuously. (6) Visible possibility had a stronger predictability to intervention effect. Intervention program had a significant difference amongst different experiemental groups.