The primary purpose of this research was to investigate the relationship among physical activity attitude, leisure satisfaction and subjective well-being of college students. The researchers also tried to understand how various gender variables affect the relationship between physical activity attitude, leisure satisfaction and subjective well-being. Convenient sampling method was adopted to survey college students from Da-Yeh University. The effective respondents were 673. Data were treated by descriptive statistics, reliability analysis, t-test, Pearson product-moment correlation, and stepwise multiple regression analysis. The results were as followings: a. The physical activity attitude of male students was significant higher than female in competition-victor aspirer, physical-adapt and exercise-skill. b. The leisure satisfaction of male students was significant higher than female in psychology factor, overall satisfaction. c. The subject well-being of female students was significant higher than male in overall life satisfaction, position emotion, negative emotion. d. There were significant and positive correlations among overall life-satisfaction and interpersonal communicative, esthetic, psychosomatic relax, physical-adapt and exercise-skill, gain effort of physical activity attitude. e. There was a positive correlation between leisure satisfaction and overall life- satisfaction, positive emotion. f. Predicting power of subjective well-being for overall leisure satisfaction, physical-adapt and exercise-skill, gain effort and interpersonal communicative was 10.5%, while "overall leisure satisfaction" possessed highest predicting power. g. Predicting power of subjective well-being for overall positive emotion, relax factor, gain effort, and interpersonal communicative was 13.0%, while "overall leisure satisfaction" possessed highest predicting power. The study suggests that physical activity attitudes, leisure satisfaction should be promoted and encouraged to the university students; and helpful promoted positive emotion and overall life satisfaction of subject well-being.