[Purpose/significance] Based on the co-opetition between business websites,this paper expounds the reasons for the formation of the information ecology chain of business network from the perspective of evolutionary game,which enriches the theoretical system of the business network information ecology chain. [Method/process] Using evolutionary game theory as a tool,as well as the replication dynamic equation,the paper analyzes the strategy selection of the nodes in the information ecology chain of business network under the influence of main factors,value factors,distribution methods and environmental factors. [Result/conclusion]Under the influence of the goal of maximizing value,business websites evolve through co-opetition relations,and ultimately makes the structure of business network information ecology chain stable.