The purpose of the study was to find out the attitude and experience ecotourism among elementary school teachers in Tainan City, also analyzed the ecotourism differences with different backgrounds. The research was conducted by questionnaire survey with the questionnaire titled "The attitude and experience ecotourism among elementary school teachers in Tainan City", questionnaires were sampling to 430 elementary school teachers in Tainan City and 410 of the samples were valid. The overall valid percentage for the questionnaire was 95%. The collected data were analyzed with descriptive statistics and one-way MANOVA by SPSS was for Windows 19.0 versions. The findings were as follows: the teachers with different years of teaching, different job positions and different amount of ecotourism participation had significant differences in the attitude toward ecotourism. The teachers of different ages, different job positions and different amount of ecotourism participation had significant differences in the experience of ecotourism. The subjects who participated more activities of ecotourism got the wanted knowledge from the scene experience and act experience, also got more significant experiences than other different backgrounds teachers, however, joint the ecotourism activities can improve the environment consciousness.