The purpose of this study is to explore the new nurses' work stress perceptive level and related factors in newly established hospital in Taiwan. A convenient sampling method was used to recruit the subjects. The samples of the study were 132 new nurses, 107 (81%) completed all questionnaires. A descriptive research design was applied for this study and the nurse stress checklist was used as the research instrument. There are four categories of stressors including 50 questions. The results were as follows. The average overall stress score for clinical nurses was 2.40-3.23(total score 4) which indicating the score represents as medium to high stress. The top 3 stressor were: (1) care with two dying patients (2) care with two critical patients (3) patients needed medical attention but doctors could not come immediately. As for the related factors, the study indicated that age, occupational status, marital status, length of working experience and religion all have influences to nurses' stress perceptive level. No statistically significant difference was found between stress and work units, level of education, past working experience and whether or not the nurse had orientation programs. This study implies that nurse leaders and administrators need to develop strategies to help clinical nurses deal with work s tress, for example, plan the stress management class for nurses; apply primary nursing care model in order to reduce stress. Designing a systemic in service training program to enhance working competency and performance of clinical nurse is also suggested.