The aim of this study was to explore the difference in depth perception between karate athletes of differing skill levels and normal college students. Methods: The subjects of this study were 67 college students who were divided into three groups according to the level of their karate ability: 22 in the "outstanding" group, 23 in the "average" group, and 22 were normal students. Their depth perception ability was tested using an electric depth perception tester and analyzed by One-way ANOVA with single sample. Differences in depth perception-25 mm/sec and 50 mm/sec (near-to-far and far-to- near) of the three groups were compared. The level of significance was set at α = .05. Results: Among these three groups, the depth perception at 25 mm/sec (near-to-far and far-to-near) and at 50 mm/sec (near-to-far and far-to-near) of the outstanding group was significantly better than the normal group (p < .05). However, at 25 mm/sec (near-to-far and far-to-near) and at 50 mm/sec (near-to-far and far-to-near), there was no difference between the outstanding and average groups, or between the average and normal groups (p > .05). Conclusions: The outstanding group had superior depth perception when compared to the college group, but differences between the outstanding and the average group, and between the average and college group, were not obvious.