The purpose of this study was to investigate whether the cooperative learning of computer-assisted instruction influences university students to learn Calculus or not. The experimental design for this study was a quasi-experimental pretest-posttest design. The 116 university students, who take in the Calculus course, were invited to participate in the study. The study adopted number of frequency distribution, independent sample t test, One-way ANOVA. The result revealed that: there were no distinguished differences in parents' education background and family economy. There were distinguished differences in learning motivation, learning achievement. Students in the cooperative learning group obtained significantly higher learning achievement of Calculus than students in the individual learning group. According to results of this study, some suggestions for designs of teaching Calculus instruction are provided in this study.