Previous studies have shown that usability of information systems may affect users’ emotion during human-computer interaction. However,studies of relationships between usability problems of information system and user emotion in terms of types and severity ratings are rather limited. To investigate the effects of types and severity ratings of usability problems on users’ emotion,the present study developed an adjective checklist of users ’ negative emotions from categorical approach and launched a two-factor mixed experiment. Results showed that users’ negative emotions elicited by usability problems included various categories and mainly involved three factors of irritable,angry,depressed and displeased. The usability problems with higher severity ratings would induce more categories and higher intensity of emotions. Types of usability problems would affect categories and intensity of users’ negative emotion,and these effects were different among different emotion categories. The interactive effects of types and severity ratings of usability problems on intensity of emotions were significant for specific categories of negative emotions. The results may support decisions on priority analysis of usability problems and users’ emotion management in human-computer interaction.