Hayao Miyazaki, a box office guarantee in Japan, Taiwan and even the US, announced his retirement after his production of the animation "Princess Mononoke," but four years later, in 2001, released his new creation "Spirited Away," which is recognized as his best work. Insisting on originality, Mr. Miyazaki himself created the story and script of "Spirited Away" and worked as the director. Widely adopting traditional Japanese Culture characterized this animation. Moreover, vivid and unique characters with a great diversity of personality and appearances are introduced in this creation. In this research, personality science and personality theories, which are both widely applied in recruiting and marketing, are applied to analyze the character design in animation. Together with the above application, interviews of Mr. Miyazaki and Studio Ghibli helped analyzing and understanding the production of "Spirited Away" and its secrets leading to success. The research wills serve as a reference or even a guideline for character design.