Softball had developed for many years in Taiwan and becomes to an important sports that could be get medals form Olympic game. In 2008 Beijing Olympic game we get the 5th. , but still had a short distance to win the medals. Therefore, Japan, American& China had done a lot of research and training about Sports Vision for many years. If we want to make medals that could form two ways to discuss, one is how to increase the softball skills, the other is try to put Sport Vision training into the daily training. The purpose of this study was to compare the Sports Vision characteristics between two groups of the female Softball players.43 female players from the Chinese Taipei softball team form two groups participated voluntarily in this study. ASICS ATHLEVISION software was be used to compare these Sports Vision Characteristics. All subject's Dynamic Visual Acuity, Eye Movement, Peripheral Vision, Momentary Vision were measured. One-way ANOVA analysis was used to analyze all the data. The result of this study was showed that senior high school group batter than college group in Dynamic Visual Acuity and Eye Movement events. But college group was batter than senior high school group in Peripheral Vision and Momentary Vision events. There had a significant result (p<.05) between two groups in Momentary Vision event. These findings suggested maybe Sports Vision Training should put into daily softball skills training program.