Based on the interpersonal theory of suicide, the stage-environment fit theory, and the escape theory of suicide, this study adopted longitudinal research design to analyze and discuss the effect of school climate and negative emotion on adolescent suicidal ideation. A total of 271 students from a middle school in Tianjin were followed up for one year(3 times in total) by three scales. The cross-lagged model was established by Mplus7.0 software, and the results showed that: 1) school climate, negative emotion and adolescent suicidal ideation all kept a high degree of stability; 2) school climate revealed the effect of negative delay prediction on negative emotion, and negative emotion revealed the effect of positive delay prediction on suicidal ideation across three time points; 3)negative emotion had a full inter-temporal mediation effect on the relationship between school climate and suicidal ideation.