Online instructor is the key person to implement e-Learning courses when higher education, enterprise's training, and relevant organizations are engaged in e-Learning courses in the future. Online instructor's role are different with the past who focused on teaching, course designer, teaching material developer, they are focused on the role of manager, developer, and facilitaror, and the e-Learning courses trend to accomplish by a team that divides the work and cooperates together. This study synthesized and summarized literatures on online instructor's competencies, and the core competencies includes e-Learning course design, online teach skill, online course management, online discusses skill, online evaluation, technology application, and online consult. In the end of the study, author refers to the online teacher's training course of Ministry of Economic Affairs and IBSTPI (International board of Standards for Training, Performance and Instruction) to suggest the online instructor's training courses in the future. Hope online instructors after training can after quality teaching advantage in online course.