While mobile devices become increasingly popular, animation has already turned to a key media material in image visionary package. Through mobile devices, animation has unknowingly penetrated our daily life. The shadow of animation usually plays a significant role in space implication and story stimulation. Aiming at computer animation application, Birn (2006) states that the major visionary functions of shadow include defining the special relation between visionary targets, enhancing tension in composition and integrating objects, etc. Space re-emergence has always been a priority in human art and visionary performance. No matter it is the artwork during Renaissance or the prevailing naked-eye 3D images nowadays, the solid space re-emergence is what being pursued mostly. This study has analysed the influence of shadow characteristics in animation on testees' perceived depth. In the experiment, a 3D animation instrument was utilized to calculate twelve images of shadow based on three independent variables - the projected lighting of shadow, the sharpness of shadow edge and picture rendering. In total, there were 47 testees, who are university students aged between 18 and 22. It is found that when the variable of whether the shadow edge is blurred or not has been considered, no matter it is studied independently or the other two variables were jointly considered - projected lighting and 2D or 3D picture rendering, the perceived depth would be affected apparently. Aiming at two groups of two-way interactions with apparent influence by deviation percentage, further analysis was conducted. The study result could be explored for the possibility of developing diversification in animation and art-related field. Also, it could be the reference or application information for educational research and pragmatic design in academic or relevant industries.