In the 64 hexagrams, every 2 ones are coupled, either upset or opposite. However, the coupled hexagrams are also have the opposite meanings, so do the 32 couples of hexagrams. In the 64 hexagrams, not only the bodies of the hexagrams are pairs, but also the meanings. Pairs are the basic characteristics of Zhouyi. The discovering of the rule that a pair of hexagrams have opposite meanings, will help us to understand the original meanings of the 64 hexagrams, the Xuguazhuan and the Zaguazhuan, and will help us to study the history of I-ching learning deeply.