The major purpose of this study was to examine whether gender and Field Dependent/Field Independent (FD/FI) learning style were the effective variables on students' learning in a virtual classroom. A paper and pencil type of post-test was administered to evaluate subjects' learning outcome. Also five variables were used to record and analyze subject's on-line learning behaviors in this study, namely, on-line frequency, averaged reading hours, total pages of reading, frequency of raising questions, and the average of on-line test scores. Study results indicated that the post-test scores were not significantly different between the FI and FD subjects. However, male subjects performed significantly better than females on the post-test. Referring to the five variables of learning behaviors, only significant difference of the total pages of reading was found between FI and FD subjects. Not any difference was found between males and females of their on-line learning behaviors.