The purpose of this research was to explore the effect of Chan-Chuang Qigong on mind-body balance of female elementary school staff. Forty one female staff members from Taipei elementary school were participated in this study. According to the quasi-experimental design and convenience sampling, subjects were divided into control (n = 21) and experimental (n = 20) groups. The experimental group was scheduled with the Qigong course, 2 times a week for 10 weeks. The researcher-designed mind-body balance questionnaire was verified with the reliability and validity tests. The ANSWatch monitor was used to collect the pre- and post heart rate variability (HRV) data. The obtained results were as follows: (1) The two-way mixed design 2 (experimental and control groups)×2 (pre- and post tests) ANOVA showed that there was an interaction between the groups and tests in the mind-body balance questionnaire. Whereas, no significant differences were found in the main effect analyses between experimental and control groups. The scores of mind-body balance questionnaire were significantly increased in experimental group, but decreased in control group. (2) Experimental group HRV measured at pre- and post tests showed no significant difference. (3) The LF/HF (low frequency/high frequency) ratio was significantly decreased in both groups (experimental from 0.96 to 0.51, control from 1.62 to 1.30). This study concludes that (1) the Chan-Chuang Qigong effect could be observed properly via mind-body balance questionnaire, (2) the Chan-Chuang Qigong was unable to improve the HRV, and (3) the Chan-Chuang Qigong could stabilize the sympathetic and parasympathetic nervous systems.