The popularity and convenience of computers have brought the prospering development of the industry in telephone customer services. For a speedy customer service, businesses mostly set up call centers for serving customers. Nevertheless, there are issues of musculoskeletal discomfort and pain experienced by people who operate computers and telephones for a longer period of time. Based upon the survey designed by Nordic Musculoskeletal Questionnaire (NMQ), this study investigated the musculoskeletal injuries of telephone customer service representatives and office workers as research subjects, in order to understand whether the their discomfort is the same as that experienced by office workers. A total of 200 questionnaires were distributed, and 182 copies were retrieved; among which 156 were valid questionnaires (84 from office workers and 72 from telephone customer service representatives). The research findings indicated that the prevalence rate of discomfort experienced by telephone customer service representative is 91.7%, much higher than 80.3% of that of office workers. Telephone customer service representatives experience significant higher discomfort with their eyes, neck, left shoulder, upper back, lower back, and waist than office workers do, especially on the left shoulder. This study suggested that more researches should be done to explore the layout of workstations and work-rest schedule for customer service representatives in order to reduce occupational hazard.