The purpose of this research aimed to develop a series of general education courses with leave-no-trace principles (short for LNT courses) and examine the effects for the promotion courses. The LNT promotion courses were executed during 4 weeks and spent 100 minutes per week. Participants were 96 undergraduates joined the pre-post test and follow-up test after a month to examine the effects of this LNT promotion courses with questionnaire of "LNT principles knowledge" as well as the scale of "environmental attitude. Statistical analysis was performed with the SPSS 12.0 for windows. According to the results, LNT courses could contribute to the improvement for LNT principles knowledge and environmental attitude. Following the results, researchers provide recommendations of future curriculum design and following environmental education program promotion.