Hematopoietic stem cell transplantation is a therapeutic strategy for various life-threatening diseases such as leukemia, lymphoma and multiple myeloma. The keystone to treatment success is effective harvest of stem cells. In our hospital, hematopoietic stem cells are collected only from peripheral blood. The process includes insertion of femoral double lumen catheters and extracorporeal circulation. Although stem cell collection is considered safe and the complications are minimal, patients or normal donors are often naïve to the procedure and therefore may have tremendous stress. The stress resulting from lack of knowledge can be improved by high-quality health education. The main purpose of this project is to improve the completeness of nursing instruction for peripheral blood stem cell collection from donors. Before implementation of this project, the completion rate of nursing instruction provided by nursing staff for peripheral blood stem cell donors was only 50.9%. Several factors account for such suboptimal results: 1). nursing personnel’s lack of awareness, 2). lack of instructional tools and 3). absence of standard operational procedures for delivering education on collection of peripheral blood stem cells. This project was implemented from December 6, 2013 to May 20, 2014. The content included: planning of in-service education programs for nursing personnel, design and application of nursing instruction progress form for collection of peripheral blood stem cells, and production and application of nursing instruction videos for collection of peripheral blood stem cells to provide health education. After this project was implemented, the completion rate of nursing instruction for collection of peripheral blood stem cells from donors increased from 50.9% to 93.3%. The goals of this project were achieved. The satisfaction of donors increased from 72.2% to 92%. In our experience, the care quality for peripheral blood stem cell donors could be improved by implementation of well-designed education programs.