Depending on Attention, Relevance, Confidence and Satisfaction (ARCS) model, the purposes of the research were to investigate the factors influencing elementary students' learning motivation in Chinese Jade Lessons of National Palace Museum (NPM) E-learning, and to understand the influences on different grade and gender elementary students. One hundred and thirty-one participants were selected from fifth and sixth grade in Taipei City and County. This research used the method of the questionnaire investigation and in-depth interview to reach the main purpose of the research. Keller's Instructional Materials Motivation Survey was adapted for the questionnaire. After finishing the Chinese Jade Lessons of NPM E-learning, the questionnaire investigation was conducted. Then, five participants were selected in order to proceed in-depth interview. In conclusion, the consequences of this study were: 1. Chinese Jade Lessons of NPM E-learning was certainly capable of improving elementary students' learning motivation; 2. There were four factors influencing elementary students' learning motivation in Chinese Jade Lessons of NPM E-learning, as following. (1) Attention: Chinese Jade Lessons of NPM E-learning contained the most popular jade-Jadeite cabbage with insects. It became the motivation to make elementary students actively to learn. (2) Relevance: Elementary students liked the course which was related to their daily lives, these were relevant to their daily lives. (3) Confidence: Chinese Jade Lessons of NPM E-learning used many lovely animation characters to narrate the course. It was not only closer to students but also made them learn easily. (4) Satisfaction: Besides plentiful and integrated course, arranging games in the course was also very important; 3. The fifth grade students were more satisfied in Chinese Jade Lessons of NPM E-learning than sixth grade students. In addition, more female students than male students considered that the Chinese Jade Lessons of NPM E-learning were combined with their life experience.