The group of junior high school students is one of the major cyclist groups. However, most of them are lacking in understanding of cycling skills and traffic rules making them one of the high-risk groups involving cycling accidents. Reference reviews reveal that the better an individual understands cycling proficiency, the safer he or she cycles. This study explored the implementation of cycling proficiency test on junior high school so as to improve their cycling safety. Investigations were performed to survey students’, parents’, and teachers’ willingness to collaborate such tests. Also, the effectiveness of cycling safety lessons appeared on students’ knowledge of cycling safety was examined and suggestion of implementing such cycling safety training program was proposed. This study was conducted on the student body of Taichung Municipal Da-Jia Junior High School. Questionnaires were distributed to the students, parents, and teachers of the school using convenience sampling technique. The result of this study shows that the students who have learned cycling safety knowledge in the classroom do demonstrate better understanding of the material than those who never studied the material and those who studied the material on their own. Prior to conducting the cycling proficiency tests, more students suggest that school should provide the test free of charge. The number of students that support holding free cycling proficiency tests increase after the tests were conducted. The parents and teachers also support that school should implement such tests but without charging cost to students.