The Occupational Safety and Health office of the hospital reported that from 2014 to 2015, sharp injuries in the operating room accounted for 30% of hospital injuries. Analysis revealed that the implementation rate of safety behavior to prevent sharp injuries by medical personnel was only 59%. The reasons were as follow: a low implementation rate of safe behavior to prevent sharp injuries, no needle-processing equipment and no safety-needle equipment, no regularly organized prevention of sharp injury education training, and no auditing mechanism. Through document verification and brainstorming of team members, diversified teaching strategies were developed on the basis of decision-matrix analysis, including adding security devices, designing slogans and audio posters, shooting and broadcasting video, planning education training, and organizing activities and regular audits. After project establishment, safety behavior implementation rate improved from 59% to 91%. Diversified teaching strategies improved staff safe behavior. These strategies can promote safety behavior among medical personnel.