Abstract The purpose of this study was to investigate the current status of kindergarten teacher’s professional knowledge and attitudes towards preschool inclusive education in Chiayi area, as well as comparing scores/differences of kindergarten teachers associated with different background variables. This study also discussed the relationship between professional knowledge and attitudes towards preschool inclusive education the targeted kindergarten teachers possessed, so as to analyze whether their attitude ratings could predict their professional knowledge ratings or not. A survey method was employed in this study. The Professional Knowledge Questionnaire of Inclusive Education and the Attitude Questionnaire of Inclusive Education were administered with the background information questions for gathering the data. There were totally 266 kindergarten teachers in Chiayi area participated in this study. Statistical methods, such as t-test, one-way ANOVA, Pearson cross-product correlation, and stepwise multiple regression, were applied for data analyses. The research findings were reported as followings: 1. The professional knowledge towards preschool inclusive education these kindergarten teachers owned was at the average level. 2. These kindergarten teachers generally held positive attitudes towards preschool inclusion education, which were at the above average level. 3. There were significant differences in their professional knowledge of preschool inclusive education among their personal background variables, such as educational backgrounds, teacher qualifications of special education, and practical backgrounds related to special education. 4. There were significant differences in their attitude towards preschool inclusive education among their personal background variables, such as educational backgrounds, previous experiences of teaching children with special needs, teacher qualifications of special education, and practical backgrounds related to special education. 5. A significant positive relationship was found between their professional knowledge of and attitudes towards preschool inclusive education. 6. The professional knowledge of preschool inclusion education these teachers possessed could predict their attitudes towards preschool inclusive education.