This research discussed the users' differences between service quality, relationship quality, customer satisfactory and loyalty of university fitness centers. Using ”The Questionnaire of Fitness Center Service Quality, Relationship Quality, Satisfaction, and Loyalty”, 437 people in six Universities in Taiwan were surveyed. The following conclusions were reached:1. Fitness Center users average a rating between satisfied and average for service quality, relationship quality, and customer satisfaction. The rating was slightly higher than the medium rating.2. Demographic variables and participant behaviors had significant difference on service quality, relationship quality, customer satisfaction and loyalty. Schools were recommended to provide more information (e.g. class schedules, activity news, etc) in order to increase customer loyalty. Subsequently, improving convenience, quantity and completeness of sports equipments, cleanliness and comfort level of the environment will aide this effort to attain the goal of encouraging students and faculty to development of exercise habits.