There has been a lot of research exploring the initiation of economic development after establishing a science park; however, they mostly applied the perspectives of strategic management and organization theory to investigate the influence after a science park is built. This study, by contrast, applies stakeholder analysis and conducts questionnaire surveys as well as interviews to examine the relationship between establishing a science park and local economic development. 200 survey questionnaires and 8 interviews, 7 of 8 both in 2004 and 2008, were conducted in this study to understand the perception of life function, mass transportation, and future economic development after Tainan Science Park has been planned and constructed. Research findings showed that the general public is satisfied with the local economic development, though most of them were not positive and hopeful as Tainan Science Park first operated. Both female and male interviewees are satisfied and positive with life function and future economic development. With respect to mass transportation, the female is more satisfied than the male and possible explanation is that most female commuters take mass transportation, while the male drive or ride motorcycles. High Speed Rail makes connection between counties much easier and more efficient, but, due to the far distance between HSR station and Tainan Science Park, shuttle service is indeed needed, which leads to moderate satisfaction. Generally speaking, promising success and positive expectation towards local economic development, life function and mass transportation after establishing the Tainan Science Park have been displayed from respondents.