Purposes: 1. to compare the differences of psychological capital, well-being and sport performance among senior high school cheerleaders with different backgrounds; 2. to explore the relationship among psychological capital, well-being and sport performance of senior high school cheerleaders; and 3. to explore the prediction of senior high school cheerleaders' psychological capital to their well-being and sport performance. Methods: The subjects were the players who joined 2015 High School Cheerleading Championship by purposively sampling. All the subjects were administered by Psychological Capital Inventory, Well-being Inventory and Sport Performance Inventory. The collected data were analyzed by t test, one-way ANOVA and multiple stepwise regression analysis. Results: 1. Male cheerleaders scored higher than female cheerleaders in self-efficacy, and the rest had no difference. 2. There were no differences between the groups in terms of "psychological capital", "well-being" and "sport performance". 3. The sport performance of the base players was better than those of the top players. 4. Self-efficacy and striving were related to positive and sport performance respectively. 5. Self-efficacy could effectively predict 13% variance of positive. Self-efficacy, striving, resilience could effectively predict 28% variance of sport performance. Conclusion: In order to enhance the feeling of well-being of cheerleaders in senior high schools, we should strengthen their self-efficacy and striving. In the prediction of sport performance, we should enhance their psychological capital (self-efficacy, striving, resilience).