The purpose of this study was to evaluate the effects of death education on the nursing students' attitudes toward death and having learned from the course. The death education course was designed as culture based. An observational study was used to explore 147 junior college nursing students through questionnaire during the final-exam. The subjects included 105 students taking death education and 42 non-taking ones. These two groups of students have high homogeneity in independent variables except the mood in answering the questionnaire on that day. The results of ANCOVA indicated that those taking death education students and those not taking had significant differences on fear of death, uneasiness about death and rejection of death. Through content analysis from students' self-evaluation we also found that they students had some changes on cognition, attitudes, behaviors and values about life and death. According to the results, the authors also gave some suggestions about the course contents and evaluations of death education on nursing students.