The aims of this study were to evaluate gender differences in the adjustment ability among newly admitted medical university students using a standardized assessment tool, and to examine the relationship between adjustment and academic performance in the future. The time scale was one year for the gender differences and differences across schools at the University. A total of 1,101 students who attended the 2003 Freshman Orientation Course completed the Psychological Adjustment Test (PAT). The relationship between the PAT results and their class rankings for academic performance in the first and second semesters were examined. The results revealed that female students showed better adjustment ability than male ones. Among these females, those who had better family and interpersonal relationships and emotional adjustment had higher class rankings for academic performance in the first semester and second semester. Among males, there was no significant association between the PAT results and their class rankings for academic performance. Interestingly, a variation in association between adjustment ability and academic performance was found among the various schools. The findings suggest that gender difference affects the adjustment ability of college freshmen and this should be taken into consideration; peer mentor support would seem to be an appropriate intervention for those in need.