This study was purposed to explore the exercise behavior and determinant factors of college students' by qualitative research (field observation and depth interviews). Five college students, who participated in regular exercise, were recruited for this study. Semi-structured interview guidelines were framed from the data of field observation and exercise theory. The transcripts were analyzed by grounded theory (open coding and axial coding), and consistency between the response and behavior of the subjects were analyzed. The results of the study included: 1. the determinant factors makes college students to begin to participate in exercise; 2. the situation of college students' exercise behavior; 3. the determinant factors which makes college students to continue participating exercise. This study concludes that 1. attitude, keep fit, cues and appropriate consideration were the factors, which affect college students to participate in exercise. 2. Perceiving benefit, over constraint behavior, exercise dependence, and social support were the factors that affect college students to persist exercise. 3. The goal of college students to take exercise was related to the behavior of exercise.