The purpose of this study is to explore the current situation and correlation of college and university PE teachers' professional belief and teacher stress. Through the self-compiled "The Study of College and University PE teachers' professional belief and teacher stress" scales, a questionnaire survey was conducted. The study subjects are 416 college and university PE teachers in the nation. The data obtained was processed using descriptive analysis, confirmatory factor analysis, MANOVA, Pearson correlation and canonical correlation analysis. The results obtained include: 1. the teachers generally demonstrated high degrees of characteristic professional belief and low levels of teacher stress, among them ethical belief had the highest rating and the students were under the most tremendous stress; 2. the teachers' professional belief and teacher stress differed due to the teachers' varied attributes; 3. the teachers' professional belief and teacher stress were significantly correlated, and both positive and negative correlation were observed; 4. the teachers' professional belief had significant influences on teacher stress, and the influences were both positive and negative.