The main purpose of this study was to develop a game-based e-learning supporting system for medical education. The learning contents were presented by means of integrating the characteristics of e-learning and on-line games. Medical knowledge from textbooks and Internet was collected and categorized in accordance with the learning objects of traditional biology curricula. Then the managed knowledge was transformed into the game mission in the design script. The game editing software used was “Well of Souls”, a shareware with low level hardware requirement from Synthetic Reality Corporation. This software engine can compose the game world not only suitable for solo but also for online multiplayer. In this study, a pre-arranged virtual environment was created for users to role-play and explore while playing in the game.