People of the Ming dynasty often targeted religious characters while probing into subjects of love and lust. Religious Prohibitions are the basic spirit of religion, and religious personages' breaking religious rules ultimately involves sex and crime. As sexual desire, like eating, is human nature, whether monks and nuns can break off all desires by practicing Buddhism or Taoism has all along gained much attention. This paper focuses on the violation of religious rule on sex in the Ming dynasty Zaju (variety plays). It examines the hardships of religious cultivation and the desire of human nature, further suggesting the hypocrisy of religious asceticism. Religious characters in the Ming dynasty Zaju involving in breaking sexual rules contain three types of roles: sheng (young man), jing (male with heavily painted face), and chou (clown). The jing and chou roles often pose as negative characters, sly and evil. As for the sheng roles, they are usually eminent monks who practice Buddhism for years but lured to break rules. In addition, Taoist and Buddhist nuns violating monastic rules are mostly played by the dan (female) roles. They go through tests of the desire during self cultivation harboring no harmful intention, thus their offense against religious disciplines are relatively slight. In conclusion, the dramatists intend to distinguish characters' images by the types of roles, accordingly these personages' personalities are closely connected to the roles applied to.